Cloned gaba receptor subtype b1a, human (hgabab1a) produced in cho cells - certificate of analysis - technical data
SignalScreen® Technical Data Certificate of Analysis
Caution: For Laboratory Use. A product for research purposes only. CLONED GABA RECEPTOR SUBTYPE B1a, HUMAN (hGABAB1a) PRODUCED IN CHO CELLS Product No.: 6110545 Please note that this is a representative technical data sheet, and that the specific lot data can vary from lot to lot. For specific information about our current lot, please contact your local PerkinElmer office. SPECIFIC LOT DATA LOT NUMBER: 6110545-01 SPECIFIC ACTIVITY: 14 pmol / mg membrane protein CONCENTRATION: PACKAGING:
50 mM Tris-HCl, pH 7.4, 10% glycerol, 1% BSA
The long term stability of 6110545 is currently being evaluated. Initial studies indicate that 6110545 can retain its original specific activity for at least 12 months when stored at -80°C in its original packaging solution.
RECOMMENDED ASSAY CONDITIONS Incubation buffer: Binding protocol:
Binding assays are performed in 540 µl total volume according to the following conditions:
1 - Membrane dilution: 0.25 ml of membranes (6110545-01) + 24.75 ml
Over GF/C filter (5 mm diam., presoaked in 50 mM Tris-HCl, pH 7.4) then washed 9x with 500 µl of ice cold 50 mM Tris-HCl, pH 7.4 at 4°C.
1PerkinElmer BioSignal uses GABA at a concentration of 10 mM
2PerkinElmer BioSignal uses [3H] CGP 54626 at a concentration of 2.5 nM for competition assays
Note: PerkinElmer BioSignal defines the unit for this receptor as the quantity of receptor protein that will yield specific binding of approximately 1,000 DPM at a concentration of radioligand close to the Kd value and using the recommended assay conditions.
BINDING DATA CLONED GABA RECEPTOR SUBTYPE B1a, HUMAN (hGABAB1a) PRODUCED IN CHO CELLS SATURATION CURVE
Typical affinity constants found with SignalScreen® hGABAB1a receptor
[3H] CGP 54626 LIMITED WARRANTY: PerkinElmer BioSignal Inc., a subsidiary of PerkinElmer Life and Analytical Sciences, warrants that, at the time of shipment, the products sold by it are free from defects in material and workmanship and conform to specifications which accompany the product. PerkinElmer BioSignal Inc. makes no other warranty, express or implied with respect to the products, including any warranty of merchantability or fitness for any particular purpose. Notification of any breach of warranty must be made within 60 days of receipt unless provided in writing by PerkinElmer BioSignal Inc. No claim shall be honored if the customer fails to notify PerkinElmer BioSignal Inc. within the period specified. The sole and exclusive remedy of the customer for any liability of PerkinElmer BioSignal Inc. of any kind including liability based upon warranty (express or implied whether contained herein or elsewhere), strict liability contract or otherwise is limited to the replacement of the goods or the refunds of the invoice price of goods. PerkinElmer BioSignal Inc. shall not in any case be liable for special, incidental or consequential damages of any kind. Worldwide Headquarters: PerkinElmer Life and Analytical Sciences, 549 Albany Street, Boston , MA 02118-2512 USA (800) 551-2121 European Headquarters: PerkinElmer Life and Analytical Sciences, Imperiastraat 8, BE-1930 Zaventem Belgium Technical Support: in Europe:[email protected] in US and Rest of World:[email protected] Belgium: Tel: 0800 94 540 • France: Tel:0800 90 77 62 • Netherlands: Tel: 0800 02 23 042 • Germany: Tel: 0800 1 81 00 32 • United Kingdom: Tel: 0800 89 60 46 Switzerland:
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SAFETY DATA SHEET Date of issue: 02.06.2003 Date to update : 24.04.2009 1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE COMPANY/UNDERTAKING 1.1. Identification of the preparation: ADHESIVES: NAUTIC MULTI 15, NAUTIC MULTI 30, NAUTIC MULTI 15W, NAUTIC MULTI 30W, NAUTIC MULTI 40, NAUTIC MULTI 45W 1.2. Use of the preparation: Adhesives NAUTIC MULTI are tw
DE LA SECRETARÍA DE GOBERNACIÓN, CON LA QUE REMITE CONTESTACION A PUNTO DE ACUERDO APROBADO POR LA COMISIÓN PERMANENTE Secretarios de la Comisión Permanente Del Honorable Congreso de la Unión Presentes En respuesta del oficio número D.G.P.L. 61-II-4-2263, signado por el diputado Heliodoro Carlos Díaz Escárraga, vicepresidente de la Mesa Directiva de la Comisión Permanen