New developments in aquatic feed ingredients, and potential of Hagerman Fish Culture Experiment Station, University of Idaho, 3059F National Fish Hatchery Road, Hagerman, ID 83332, USA ABSTRACT: Aquaculture production has expanded at a rate of 15% per year and is predicted to continue to
grow at this rate for at least the next decade. Demands on traditional fish feed ingredients, mainly fish meal
and oil, which are finite global resources, are increasing. At present, global fishmeal production averages
6.5 mmt per year, of which 23% is utilized in feeds for farmed fish. Global fish oil production averages 1.4
mmt per year, and 25% of this yearly production is utilized in fish feeds. Up to now, 70% of the fish meal
and oil used to produce farmed fish has been consumed by salmon, trout and shrimp, despite the fact that
these species account for only 30% of global fish feed production and only 7% of global aquaculture
production. Clearly, expanded production of carnivorous species requiring high protein, high-energy feeds
will further tax global fish meal and oil supplies. Suitable alternative feed ingredients will have to been
utilized to provide the essential nutrients and energy needed to fuel the growth of aquaculture production.
Rendered products, seafood processing waste, including by-catch, and grain and oilseed by-products are the
most likely candidate feed sources to carry aquaculture forward to higher production levels. Worldwide,
annual production of rendered products is roughly equivalent to annual fish meal production, with meat and
bone meal and poultry by-product meal making up 80% of total production. These products are variable in
quality, high in ash content, and fully utilized by other agricultural sectors. They are unlikely to supply a
high proportion of the protein needed in fish feeds, but may be valuable as feed components due to their
favorable amino acid profiles, which complement plant-derived protein sources. If seafood processing waste
and by-catch were converted to fish meal, the quantity would nearly equal annual global fish meal
production and potentially provide significant fish protein and oil supplies for aquaculture feeds. However,
the high ash content and logistical problems with collection and processing will limit full utilization of this
resource. Grain and oilseed by-products are thus the most promising sources of protein and energy for
aquaculture feeds of the future. Despite many successful research studies on the use of plant-derived feed
ingredients in fish feeds, significant problems remain to be resolved. Innovative collaborative research
efforts between geneticists, fish nutritionists and the industrial sectors producing these products are
beginning to resolve these technical problems. Use of enzyme supplements is one potential aspect of
alternate ingredient utilization that will increase the nutritional value and use of alternate feed ingredients.
KEY WORDS: enzyme supplement, animal by products, grain protein, oilseed protein.


Fish meal production has averaged approximately 6.5 million metric tons (mmt) over the past decade,
and prospects for this production to increase are low. The highest annual production of fish meal has
been 7.5 mmt, and the lowest production was between 4.5 and 5.0 mmt, during the 1998 El Niño
1 Hardy, R.W., 2000. New developments in aquatic feed ingredients, and potential of enzyme supplements. In: Cruz -Suárez, L.E., Ricque- Marie, D., Tapia-Salazar, M., Olvera-Novoa, M.A. y Civera-Cerecedo, R., (Eds.). Avances en Nutrición Acuícola V.Memorias del V Simposium Internacional de Nutrición Acuícola. 19-22 Noviembre, 2000. Mérida, Yucatán, Mexico. period, which lowered production of fish meal from Peru and Chile. Since Peru and Chile have
accounted for about 1/3 of global fish meal production, any change in these countries has a major impact
annual production. Further, Peru and Chile are major fish meal exporting countries, accounting for up
to 2/3 of the amount of fish meal traded throughout the world. Thus, production of fish meal by Peru
and Chile greatly influences the supply of fish meal, which in turn affects fish meal price (Fig. 1). The
price of fish meal is currently quite low, the result of adequate supplies and relatively low demand, most
likely associated with the economic slowdown in Asia. However, this period of low fish meal prices is
likely to be short-lived, in that economic recovery in Asia is underway, and production of fish meal in
Chile is not expected to recover to pre-El Nino levels in the next few years (Fig. 2). The aquaculture
industry must be prepared for higher feed costs, associated with higher fish meal costs, and in addition
must seek alternative protein sources to replace a portion of the fish meal in feed formulations to permit
expansion of aquaculture production beyond the level at which supplies of fish meal become a factor
limiting production of fish feeds, and hence farmed fish.

Figure 1.- Average fish meal price from 1990 to 1996 86 87 88 89 90 91 92 93 94 95 96 97 98 99 Figure 2.- Production of fish meal from Chile and Peru between 1986 to 1995
Fish meal use in aquaculture feeds

The proportion of global fish meal production that is utilized in fish feeds has increased substantially
over the past 10 years. In 1989, aquaculture was a minor consumer of fish meal, using approximately
10% of annual production (Barlow, 1989). In 2000, fish meal consumption by the aquaculture industry
will be an estimated 35% of total global fish meal production, an increase 3.5 times in fifteen years.
Growth of the Atlantic salmon and shrimp farming industries has been responsible for most of the
increase in fish meal use by the aquaculture industry over this period, but the explosive growth of the
marine fish farming industry has caused much of the increase in fish meal use by aquaculture in the last
five years (Fig. 3). Feeds for Atlantic salmon over the past 15 years have contained more than 50% fish
meal, and shrimp feeds 35% fish meal (Barlow, 2000). In the past five years, these percentages have
decreased somewhat, but nevertheless, feeds for salmon, marine fish, and eels still contain about 40%
fish meal (Barlow, 2000). Predictions of fish meal needs for aquaculture feeds in 2010 are 2.83 mmt,
approximately 44% of the ten-year average annual global fish meal production of 6.5 mmt. This
represents an increase of 716,000 mt over estimates of fish meal use in 2000. Fish meal use in feeds for
carp is predicted to increase by 325,000 mt, and use for marine fish by 447,000 mt, while use in feeds
for eels, salmon, trout, milkfish, and catfish is predicted to decrease (Table 1). Use of fish meal in
shrimp feeds is predicted to increase from 372,000 mt to 485,000 mt between 2000 and 2010. The
percentage of fish meal in feeds for all species groups is predicted to decrease (Table 2). If the
percentage of fish meal use in fish feeds was to remain the same as today, and aquaculture production
increased to predicted levels in 2010, fish meal needs would be 4086 mmt, or 63% of the average
amount produced over the past decade. The difference between the predicted need for fish meal by the
aquaculture feed industry in 2010 (2.83 mmt), and the amount that would be needed if the percentage of
fish meal in fish feeds did not decrease (4.086 mmt) is 1,255,000 mt. This is the amount of fish meal-equivalent protein sources that will be needed to replace the ‘missing’ fish meal in fish feeds by the year 2010. Figure 3.- predicted fish meal use in 2000 and 2010 by sector Table 1. Amount of fish meal (1000 mt) used in fish feeds in 2000 and estimated for 2010. Table 2. Percent fish meal used in fish feeds in 2000 and estimated for 2010.
Alternative Protein Sources; Availability and Quantity

Seafood Processing Waste and By-Catch

Seafood processing waste and fishery by-catch together exceed in tonnage the global landings of fish for
fish meal production (New, 1996). If half of the fishery by-catch discarded by the fishing industry each
year could be converted into fish meal, this quantity (2,600,000 mt) could supply the expected needs of
the aquaculture feed industry for the next 15 years or more. Seafood processing waste, which is mainly
the carcass of fish after fillets are removed, contains too much bone to be producing suitable fish meal
for fish feeds. Therefore, the bone content of the processing waste must be reduced, either before it is
made into fish meal by mechanical de-boning, or after it is made into fish meal by screening (Babbitt et
. 1994). Fish processing waste contains ca. 25% ash on a dry weight basis, but fish meals made from
de-boned fish filleting waste can be as low as 7% ash, half the level of ash in fish meals used in feeds.
This is particularly valuable for the production of low-pollution fish feeds. Expanded production of low-
ash fish meals produced from seafood processing waste is likely, as is further refinement of the
production process to ensure that the nutritional value of these fish meals remains high.

Rendered Products

Rendered products are meat & bone meal (annual US production 2,819,322 mt) and blood meal (annual
US production 101,300 mt). By-products of poultry processing include feed grade poultry by-product
meal (annual US production 265,910 mt), pet food grade poultry by-product meal (annual US
production 177,270 mt), low-ash pet food grade (annual US production 24,000 mt), and feather meal
(annual US production 363,640 mt). Together, annual US production of all rendered products plus
poultry processing products totals 3,751,442 mt, or about 50-60% of average annual world fish meal
production. These products are fully utilized in poultry feeds, pet foods, and other animal feeds. At
present their prices are very low in comparison to 10-year average prices, both on a weight basis and on
a protein-unit basis (Table 3).
Table 3. Current prices (October, 2000) and production levels of protein sources used in fish feeds. Rendered products have not been extensively studied as replacements for fish meal in feeds for carnivorous fish. Dong et al. ((Dong et al. 1993) reported that poultry by-product meal varied considerably in quality among suppliers, as measured by apparent protein digestibility. Feeding trials involving poultry by-product meals have demonstrated that up to 40% of fish meal could be replaced with pet-food grade poultry by-product without lowering trout growth, but that higher replacement levels resulted in reduced growth. Protein digestibility of poultry by-product meal, measured in trout, is 94-95%, equivalent to herring meal (Sugiura et al. 1998a) but recent research results suggests that the availability of certain amino acids in poultry meal is lower than average protein digestibility. Meat and bone meal and feather meal were considered to be unsuitable for use in salmonid feeds because early data showed less than 70% protein digestibility (Cho and Slinger, 1979). Recent re-evaluation of several of these ingredients has shown that earlier work underestimated the protein digestibility of meat & bone meal and blood meal, with more recent values showing apparent digestibility coefficients ranging from 87% to 92% (Hajen et al. 1993; Sugira et al. 1998b, Bureau et al. 2000). As is the case with poultry by-product meal, individual amino acid digestibility coefficients are higher and lower for specific essential amino acids than the average protein digestibility value for meat and bone meal (unpublished data, Sugiura and Hardy, 1998). Initial studies suggest that up to 25% of fish meal protein can be replaced with meat and bone meal without compromising growth, but that higher levels of replacement significantly reduce growth (Schelling and Hardy, unpublished data, 2000). The nutritional value of rendered products varies among producers, and even among manufacturing plants owned by the same company. The most important determinate of nutritional value is the source and freshness of the raw material used to produce the meals. At present, rendered products are sold at commodity prices, but efforts are being made within the rendering industry to range products and establish grades corresponding to nutritional value. Grain Proteins

Wheat gluten is an excellent protein source, containing 70-80% protein that is highly digestible to
rainbow trout, coho salmon and presumably other fish species (Sugiura et al. 1998). Up to 25% of fish
meal has been replaced with wheat gluten without negative effects on growth or feed conversion ratios
(Weede, 1997). Higher replacement levels combined with lysine supplementation are reported to
support trout performance equivalent to fish meal-based diets (Rodehutscord et al. 1994). The main
drawback of wheat gluten is its relatively high price. Wheat gluten is currently produced for human
consumption as a high-value, non-meat protein source. If lower quality, cheaper, feed-grade wheat
gluten were developed, this ingredient could become an important aquaculture feed ingredient.

Corn gluten is an excellent protein source, containing a minimum of 60% protein (Morales et al., 1994)
which is 97% digestible to trout (Sugiura et al. 1998). Corn gluten can substitute for 25–40 % of fish
meal without negative effects on growth or feed conversion ratios in trout (Morales et al., 1994; Weede,
1997). The main disadvantage of corn gluten for commercial trout diets is that it imparts a yellow color
to fish flesh when included at a high proportion of the diet (Weede, 1997). Nevertheless, it is a valuable
ingredient when included at levels up to 10% in trout diets, and, when trout or salmon are raised with
the intention of producing fish with pink colored flesh, corn gluten can be included as up to at least
22.5% of the diet, along with canthaxanthin or astaxanthin, which masks the yellow color in fillets
(Skonberg et al. 1998). For fish species that do not deposit carotenoid pigments in their flesh, corn
gluten can be used at even higher dietary levels. Corn gluten has the advantage of being plentiful and
low priced. In 1997, U.S. production alone amounted to 1.178 mmt, and was priced at $380 per mt.
Currently, corn gluten produced from white corn is being evaluated as a feed ingredient for salmonids,
and initial results appear promising (Hardy, unpublished data, 2000). White corn gluten meal will likely
be priced at a premium to regular (yellow) corn gluten meal, but protein levels are at least 10% higher,
which justifies the increased cost.

Oilseed Proteins

Soybeans, as other plant-derived protein sources, have several antinutritional factors (ANFs), which can
reduce palatability, protein utilization or growth (Hardy, 1996). These can be divided into two
categories: heat-labile and heat-stable ANFs (Rumsey et al. 1995). Heat-labile ANFs include trypsin
inhibitors, phytates, lectins, goitrogens and antivitamins. Heat-stable ANFs include carbohydrate or
soluble fiber, saponins, estrogens, allergins, and lysinoalanine. Heat-labile constituents can be at least
partially degraded by heat treatments, so the effects of these antinutritional factors reduced by adjusting
the heat treatment used during soybean presscake drying (Vohra and Kratzer, 1991). Trypsin inhibitors
decrease the activity of trypsin, a digestive enzyme that breaks down proteins in the intestine. Trypsin
inhibitors lower protein digestibility in diets for salmon and trout (Arndt et al., 1999). Phytate or phytic
acid has been reported to reduce protein digestibility and limit the bioavailability of minerals (Spinelli et
. 1983; Riche and Brown, 1996). Much of the phosphorus in plant-derived ingredients is bound in
phytate. Supplementing the diet with the enzyme phytase can break down a portion of the phytate,
increasing availability of dietary phytate-phosphorus in diets for fish (Rodehutscord and Pfeffer, 1995;
Schafer et al., 1995; Cain and Garling, 1995). Soybean lectins have been shown in vitro to bind to the
brush border membrane of Atlantic salmon small intestine (van den Ingh et al. 1991), but no studies
have been conducted on performance or health effects of lectins on fish. The carbohydrate fraction of a
soybean is approximately 30% of its dry weight, and only 33% consists of the soluble fraction
(oligosaccharides, raffinose, sucrose, and stachyose), or the fraction available for energy use (Arnesen et
. 1989). Arnesen et al. (1989) suggested that a large fraction of the potential carbohydrate energy is
not available to salmonids because most of the soybean polysaccharides cannot be absorbed. This
carbohydrate fraction is unavailable because salmonids only have the enzyme necessary to digest starch
and starch makes up less than 1% of soybean meal. A crude saponin extract of soybean meal was found
to lower feed intake of chinook salmon fingerlings and to reduce growth of rainbow trout (Bureau et al.
1996). Overall, there are several ANFs that could influence the nutritional value of soybean meal and
other plant-derived ingredients for fish; additional processing or diet supplementation may be required to
realize the full nutritional potential when these ingredients are used in fish feeds.
Soybean products are generally high in protein, ranging from about 45% protein for soybean meal to
over 70% protein for soy protein concentrate. Soybeans are the most plentiful of oilseed crops, with a
worldwide production of 132.53 mmt in 1996. US production of soybean meal in 1996 was 30.6 mmt,
and the price was $289 per mt. Soy protein concentrate is produced in smaller quantities, with US
annual production of 85,000 mt, and is priced at about $990 per mt. Early studies with soybean meal in
trout feeds showed that trout tolerated relatively high levels of soybean meal in their feeds, especially if
the meal was heat-treated to inactivate trypsin inhibitor levels (Cho et al. 1974; Reinitz, 1980). Studies
with Pacific salmon fingerlings, however, are less promising, with some studies showing that feed intake
was reduced even at 5% soybean meal in the diet (Higgs et al. 1979; Fowler, 1980). Recent studies with
post-juvenile Pacific salmon have been more encouraging, suggesting that larger fish are more tolerant
of soybean meal in their feed than are fry and fingerlings. Wilson (1992) found that full-fat soybean
meal, heat-treated by double extrusion to lower trypsin inhibitor levels, could be used in diets for post-
juvenile chinook salmon at levels up to 15% of the diet without reducing growth rates or feed efficiency
ratios, but that diets containing more than 15% full-fat soybean meal resulted in reduced feed intake and
growth. Further research is necessary to determine whether higher levels of soybean meal can be
included in diets for salmon when appetite stimulants are included in the diet, and to determine the
relative importance of various antinutritional factors in soybean meal for fish. In contrast to salmon and
trout feeds, catfish feeds depend heavily on soybean meal to provide dietary protein. Current catfish
feed formulations in the US contain 45-50% soybean meal, with less than 10% fish meal (Wilson, 1991).
Similarly, tilapia and carp feeds generally contain less than 15% fish meal, with soybean meal or other
alternate protein sources providing the bulk of dietary protein (Luquet, 1991) (Satoh, 1991).

Other By-Product Proteins

By-products of the brewing and distilling industries are widely available and underutilized in feeds for
fish. Rumsey et al. (1991) found that the protein quality of Saccharomyces yeast (brewers or bakers
yeast) in diets for rainbow trout is improved by a treatment to disrupt the cell walls, thereby making the
protein more available. When yeast cell walls are disrupted, 50% of the protein in rainbow trout diets
can be supplied by bakers yeast with equivalent growth and feed conversion ratio to a control diet with
protein supplied by casein and gelatin. Although single cell proteins are potentially good protein
sources, limited availability or high cost has so far limited their use in fish diets. This situation may
change in the near future, however, as new processes are developed to recover single cell proteins from
brewery waste, and upgrade its quality by air-classification to lower fiber content.

Role of Enzyme Supplements

As fish meal is increasingly replaced in fish feeds with non-traditional protein sources, the opportunity to
improve the nutritional value of these protein sources by enzyme supplementation will increase. Phytase
is already used in swine and poultry feeds to increase phosphorus availability in grains and oilseeds by
dephosphorylation of myo-inositol hexakisphosphate (phytate)) (Cromwell et al. 1993). Studies with
catfish (Jackson et al. 1996; Eya and Lovell, 1997; Li and Robinson, 1997) and trout (Cain and Garling,
1995; Rodehutscord and Pfeffer, 1995; Vielma et al. 2000) demonstrate the effectiveness of phytase at
increasing phosphorus availability in fish, although these studies also demonstrate the significance of
rearing water temperature on effectiveness and optimum dietary phytase level. Li and Robinson (1997)
found that the cost of adding phytase to catfish feeds was nearly equal to the savings associated with
eliminating dietary supplementation with inorganic phosphorus.
Other enzyme supplements are not widely used, but may be added to future fish feeds to increase
nutritional value when alternate ingredients are included. For example, mixtures of proteases may be
used increase the digestibility of protein in rendered products. Such products would contain enzymes
that hydrolyze connective tissue and skin, two components of rendered products that are difficult for fish
to digest. Another category of enzyme supplements is those that break down fiber and certain
carbohydrates found in protein sources from grains and oilseeds. One such product, designed specifically
for use in high-wheat feeds for poultry, contains endo-xylanase, which breaks down pentose sugars. A
similar product breaks down glucans found in wheat, barley, triticale and rye, releasing glucose. To
date, these products have been only used in poultry and swine diets, but it is likely that they will be
effective in diets for tilapia, catfish, and perhaps shrimp.
Specific enzyme supplements are needed to overcome various components of the carbohydrate fraction
of oilseeds. As mentioned above, soybean non-starch polysaccharides are suspected of being one of the
problems that limits soybean meal nutritional value for some species of fish. Studies in poultry show that
supplementing feeds with a glycanase increases the performance of the birds when their diet contained
low metabolizable energy wheat (Choct, Hughes, et al. 1995 #20771). Supplementation with the
enzyme significantly increased solubilization of non-starch polysaccharides in the intestine of the birds.
Enzymes that break down non-starch polysaccharides must be tested in fish to determine if nutritional
value, specifically energy availability, is increased in soybean meal-containing diets when enzyme
supplements are used.


Expanded aquaculture production will require more fish feed, which will in turn require higher quantities
of alternate protein sources to substitute for fish meal. An estimated 1.5 mmt of alternate proteins will
be needed just in the next decade to supply global needs. If fish meal supplies decrease, higher amounts
will be needed. Most likely, these proteins will be supplied from a variety of sources, most of which
requiring special processing or enzyme supplementation to realize their full nutritional value. The
aquaculture industry should look to blends of protein sources from plant sources and from animal or fish
sources. Such blends would more closely approximate the excellent amino acid profile of fish meal that
any single protein source, with the exception of fish meal produced from seafood processing waste.


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Soy protein in diets of rainbow trout: Effects on growth, protein absorption, gastrointestinal histology and nonspecific serologic and immune response. In C.E. Lim & D.J. Sessa (Eds.), Nutrition and Utilization Technology in Aquaculture (pp. 166-188). Champaign, IL: AOCS Press. Rumsey, G.L., Hughes, S.G., Smith, R.R., Kinsella, J.E., Shetty, K.J., 1991. Digestibility and energy values of intact, disrupted, and extracts from brewers dried yeast fed to rainbow trout (Oncorhynchus mykiss). Anim. Feed. Sci. Tech., 33, 185-193. Satoh, S., 1991. Common Carp, Cyprinus carpio. In R.P.Wilson (Ed.), Handbook of Nutrient Requirements of Finfish (pp. Schafer, A., Koppe, W.M., Neyer-Burgdorff, K.H., Gunther, K.D., 1995. Effects of microbial phytase on utilization on native phosphorus by carp in diets based on soybean meal. Water Sci. Tech., 31, 149-155. Skonberg, D.I., Hardy, R.W., Barrows, F.T., Dong, F.M., 1998. 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Trabajos de autores extranjeros • Traducción realizada por la Dra Claudia Rodari con autorización de las partes involucradas Recomendaciones y bases para el tratamiento de la enfermedad pelviana inflamatoria Oluwatosin Jaiyeoba, Gweneth Lazenby, David E Soper Department of Obstetrics and Gynaecology, Medical University of South Carolina. Expert Rev Anti Infect Ther 2011;9(1):61-70 Rep


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