Pharmacological screening of root of

Acta Pharmaceutica Sciencia
48: 11-17 (2006)

Pharmacological Screening of Root of Operculina turpethum and
its Formulations
2, Pramod Kumar2, Nitin K. Mahurkar3 and
S. Ramachandra Setty1*
1 Department of Pharmacology, S.C.S. College of Pharmacy, Harapanahalli-583131 India 2 Department of Pharmacognosy, V.L. College of Pharmacy, Raichur-584 103. India 3 Department of Pharmacology, H.K.E.’s College of Pharmacy, Gulbarga - 585 101 Abstract

The root of Operculina turpethum is the chief ingredient in the Ayurvedic formulation viz. Avipattikara
churna used for the treatment of gastric ulcer and related gastrointestinal disturbances. Its incorporation in
the formulation is not scientifically studied. Hence, in the present study root and its formulations are
investigated for anti-ulcer, anti-inflammatory and anti-diarrheal properties.
The root powder of Operculina turpethum and its formulations were studied for anti-ulcer activity by
using Shay rat that have administered preparation at 30 mg/kg and 100 mg/kg dose levels. The results
revealed that the root powder and formulations have reduced the hyperacidity to the extent of 50 – 55% at
100mg/kg dose level. The root powder was found to be better than all formulations. The root powder and
the formulation have reduced the charcoal movement significantly in charcoal meal test in mice at
400mg/kg dose. Similarly, these preparations have reduced the edema volume in formalin induced
inflammation model in rats at 100mg/kg dose.
The study revealed that the incorporation of root of Operculina turpethum in Avipattikara Churna is
justifiable.
Key words: Avipattikara churna, diarrhea, edema volume, hyperacidity, Operculina turpethum,
reduction and ulcer.

Introduction

Operculina turpethum is a perennial herb with milky juice and its root is incorporated in the
Avipattikara churna (an Ayurvedic preparation used for the treatment of hyperacidity, gastric
ulcer and related gastrointestinal disturbances). The formula of the Avipattikara churna is given
in table 1. This churna is useful in the treatment of Amla Pitha (Hyperacidity), malabandha
(Constipation) (Ayurvedic formulary of India, 1976). In ancient literature it is named Trivrit
(Ogle, 1931) Operculina turpethum is the chief ingredient in the formulation. Zandu
Pharmaceuticals, Bombay, India, Sandu Pharmaceuticals and many native practitioners are
manufacturing and marketing the same product with generic names. Upon literature review, it
was found that the active principle of the plant is glycosidic resin (Ambasta, 1986). The
scopoletin, a coumarin derivative, turpethinic acid and its derivatives were isolated from the
plant (Rastogi and Mehrotra, 1999). However, there are no reports regarding its
pharmacological profile in the modern literature. Hence, in the present study it was planned to

* Corresponding author : e-mail: [email protected]
investigate the claims of the Ayurveda regarding the usage of Operculina turpethum and the
Avipattikara Churna in treating hyperacidity and related gastrointestinal disturbances.

Materials and methods

Collection of Plant material
The root of the plant was obtained from crude drug market and was identified by the native
practitioner who is prescribing the preparation for his patients and was further identified by
Prof. Srivatsa, Head, Dept. of Botany, L.V.D. College, Raichur, India. Thus obtained plant
material was powdered and stored in an airtight container. Similarly Marketed Avipattikara
churna was purchased from the Ayurvedic medical hall and a formulation was obtained from
the native practitioner himself.

Table–1: Formula of Avipattikara Churna
Each 10 g contains:
Sl. Name of Binomial name Quantity
No. ingredients in Gm
01. Sunthi Zingeber officinalis 0.075 02. Kali mirchi Piper nigrum 0.075 03. Pippli Piper longum 0.075 04. Tiphala i. Amlaki Emblica officinalis 0.075 ii. Haritaki Terminalia chebula 0.075 iii. Bibhitaka Terminalia bellerica 0.075 05. Mustha Cyprus rotundeus 0.075 06. Varading Embelia ribes 0.075 07. Ellyachi Elettaria cardamomum 0.075 08. Lavang Syzygium aromaticum 0.833 09. Patra Cinnamomum tamala 0.075 10. Vida lavana Sod. Sulfate 0.075 09. Trivrit Operculina turpethum 3.333 10. Sugar - - - 5.000 Animals: Acute toxicity and blind screening studies were carried out in healthy mice of either sex weighing between 25 – 35g. The anti-inflammatory and anti-secretory and anti-ulcer activities were studied in healthy rats of either sex weighing between 150-200g. Both species were obtained from the central animal house, V.L. College of Pharmacy, Raichur. The animals were randomly distributed into various groups and were kept in colony cages at ambient temparature of 270 ± 20 C and at 45 – 55% relative humidity with a 12 hour light /12 hour dark cycle. Blind Screening and Acute toxicity studies The screening method as described by Robert (1965) was adopted in the present study. Albino mice of either sex weighing between 25–35 g were fasted and divided into 29 groups of 6 animals each. All the animals were fasted for 24 hours prior to the experiment. Animals of the group 1 received acacia suspension (0.5 ml orally). The animals of group 2 to 8 received suspension of root of Operculina turpethum (OT) at 10, 30, 100, 200, 400, 600, 800 mg/kg dose levels respectively. Similarly animals of group 9 to 15 received AVIFN (Avipattikara Churna from native practitioner), animals of group 16 to 22 received AVIFS (Avipattikara Churna from Sandu Pharmaceuticals) and animals of group 23 to 29 received AVIFZ (Avipattikara Churna from Zandu Pharmaceuticals) respectively at the dose levels mentioned above. The animals were observed at 0, ½, 1, 2 and 4 hours after the administration for acute effect and mortality. The observation was continued for one week for the delayed effects and mortality. However there were no deaths in any of the groups. Antisecretory and Ulcer protective activity Albino rats of either sex weighing between 150 – 200 g were divided into 11 groups of 6 animals each. These animals were fasted for 24 hours and water ad libitum, prior to experiment. Animals of groups 1 and 2 received 5% acacia suspension and omeprazole 30 mg/kg respectively. Similarly animals of groups 3 and 4 received Operculina turpethum 30 mg/kg and 100mg/kg, respectively. The animals of groups 5 and 6 received AVIFN, 7 and 8 received AVIFZ, 9 and 10 received AVIFS equivalent to 30mg/kg and 100 mg/kg of Operculina turpethum orally, respectively. The animals of group 11 received 100 mg/kg of Avipattikara churna (AVIFN) without Operculina turpethum (AVIFN – OT). One hour after the administration of the drugs, ulcers were induced by pyloric ligation (Shay et al., 1945). After six hours the animals were sacrificed by decapitation. Abdomen was opened, oesophageal end was tied and the stomach was isolated. The contents of the stomach were collected and gastric volume, free acid and total acid content were estimated by titrimetric method. The stomach was opened; ulcer index was determined (Kulkarni, 1987) by observing under 10X lens and by giving scores (normal colored stomach = 0, red coloration = 0.5, spot ulcers = 1, hemorrhagic streaks = 1.5, ulcer ≥ 3 but ≤ 5 = 2 and ulcers ≥ 5 = 3). The results are compiled in table 2. Anti-diarrheal activity Mice of either sex weighing between 20 – 30 g were divided into 4 groups of 6 animals each. These animals were fasted for 24 hours prior to experiment. Animals of group 1 received 0.5 ml of 5% acacia suspension, group 2 received loperamide HCl 5 mg/kg, and groups 3 and 4 received Operculina turpethum and AVIFN 400 mg/kg orally, respectively. One hour after the administration of drugs 0.3 ml of 5% charcoal suspension (charcoal meal) was given to all the animals (Robert, 1965). Fifty minutes after the charcoal meal, all animals were sacrificed by decapitation. Abdomen was opened and the total length of the intestine was measured. The distance traveled by the charcoal meal in the intestine was also measured. The % movement of the charcoal meal in the intestine with reference to the total length of the intestine was calculated. The results are compiled in table 3. Anti-inflammatory activity The albino rats of either sex weighing between 120 – 160 g were selected and divided into 6 groups of 6 animals each. The animals were fasted for 24 hours prior to the experiment. Animals of groups 1 and 2 were treated with 0.5 ml of 5% acacia suspension and diclofenac sod., 30mg/kg respectively. Similarly, animals of groups 3, 4, 5 and 6 were treated with Operculina turpethum, AVIFN, AVIFS and AVIFZ 100mg/kg respectively. One hour after the
drug treatment all animals of all groups were given 0.1 ml of formalin subcutaneously into the
subplantar region of the left hind paw. Paw volumes were measured at 0, ½, 1, 2, 4, 6, 8, 12, 18
and 24 hours after the formalin challenge by using plethysmograph (Kulkarni, 1987). Edema
volumes were calculated and % reduction in the edema volume due to drug treatment was
determined by comparing with control group (i.e. group1). The results are compiled in table 4.

Results and Discussion
All the preparations studied have shown dose dependent stimulation in the animals however the
plain OT was found to be more effective among all the preparations. None of the preparations
were found to be toxic in mice up to the dose of 800 mg/kg. And hence, LD50 of the
preparations could not be calculated. But it is presumed to be above 800mg/kg dose. 30mg/kg
and 100mg/kg doses have shown significant activity in blind screening, therefore these doses
were opted for further studies.
All preparations viz. OT, AVIFN, AVIFS and AVIFZ were found to reduce the acid content
(both free acid and total acid), but failed to reduce the volume and ulcer index significantly with
single bolus injection in Shay rat with the preparations at both dose levels tried i.e. 30 mg/kg
and 100 mg/kg. OT was found to be more effective in this case also. Since, LD50 is more than
800 mg/kg and is 5 times more than that of effective dose (100 mg/kg), we can infer that the
root powder and its marketed formulations are safe for treating hyper acidity and gastric ulcer.

Table–2: Antisecretory and Ulcer protective activity of Operculina turpethum

Drug Dose Free acid Total acid Acid volume Ulcer index
Mg/kg mEq/l/100g mEq/l/100g ml/100g
5% Acacia 0.5 ml 84.4 ± 1.2 90.8 ± 1.9 3.9 ± 0.1 4.6 ± 0.2 suspension (Control) Omeprazole 30 04.9 ± 0.4* 07.2 ± 0.3* 1.2 ± 0.1* 1.3 ± 0.2* (Std.) OT 30 55.2 ± 0.4* 59.9 ± 0.8* 3.1 ± 0.3 3.6 ± 0.2 100 48.5 ± 0.5 52.2 ± 0.5 3.0 ± 0.4 3.5 ± 0.2 AVIFN 30 67.3 ± 1.3* 73.0 ± 1.2* 4.7 ± 0.3 3.8 ± 0.3 100 53.1 ± 0.6* 57.6 ± 0.4* 4.8 ± 0.2 3.5 ± 0.2 AVIFS 30 62.8 ± 0.5* 66.4 ± 0.5* 4.1 ± 0.2 3.6 ± 0.3 100 54.3 ± 0.3* 60.0 ± 0.3* 3.6 ± 0.2 3.5 ± 0.2 AVIFZ 30 72.7 ± 2.9 81.0 ± 3.0 6.9 ± 0.3 4.3 ± 0.2 100 63.9 ± 2.7* 69.4 ± 2.1* 4.8 ± 0.3 3.6 ± 0.2 AVIFN – OT 100 81.8 ± 0.5 85.6 ± 0.7 6.8 ± 0.5 4.3 ± 0.2 Pilot studies on charcoal meal test in mice revealed that lower doses like 30 mg/kg, 100 mg/kg and 200 mg/kg could not influence the intestinal movement. Hence, 400mg/kg dose was selected. Single bolus injections of OT and AVIFN have reduced the intestinal movement from 75.1 ± 1.1 to 56.0 ± 1.5 and 67.5 ± 2.1 respectively. The results of charcoal meal test reveals that, the dose required was higher (400mg/kg) than the dose required for reducing the hyperacidity. In addition the exact LD50 of the OT/formulations are not established. Therefore, using these preparations for antidiarrheal purpose require further studies to confirm their safety. Table–3: Antidiarrheal activity of Operculina turpethum & Avipattikara churna
Drug Average Average length % movement
Total length moved by marker of marker
of git (in cm) (in cm)
Control 51.92 ± 1.77 39.25 ± 1.44 75.1 ± 1.1
5% Charcoal
suspension
Loperamide HCl 64.00 ± 2.28 28.50 ± 1.33 44.5 ± 1.6*
5 mg/kg Std.
OT 59.08 ± 2.87 32.92 ± 0.88 56.0 ± 1.5*
400 mg/kg
AVIFN 64.00 ± 1.31 43.17 ± 1.19 67.5 ± 2.1*
400 mg/kg
* Significant at P=0.05
The pretreatment with the root powder of OT, and marketed Avipattikara churna viz. AVIFN, AVIFS and AVIFZ (100 mg/kg dose) have reduced the formalin induced edema volume to the extent of 36.45%, 27.11%, 18.69% and 21.15% respectively. All these preparations have demonstrated significant anti-inflammatory activity; therefore they are useful in hyperacidity and ulcer related bowel inflammation also Table – 4: Antiinflammatory activity of Operculina turpethum and Avipattikara churna

Drug Mean % reduction in edema volume
½ hr 1 hr 2 hr 4hr 6hr 8hr 12 hr 18 hr 24 hr

Control - - - - - - - - - - - - - - - - - -
0.5ml 3%
acacia
suspension
Diclofenac 21.0±9.6 29.2±7.5 44.5±6.5 60.4±3.8 36.3±5.2 29.0±6.3 25.7±4.9 21.3±4.3 23.3±5.0
Sod. Std.
30 mg/kg
OT 11.4±4.0 12.9±2.8 25.7±7.0 36.4±8.5 28.7±7.5 20.1±5.5 15.7±4.3 19.0±4.9 28.5±8.9
100 mg/kg
AVIFN 08.9±5.6 10.8±7.1 17.8±4.8 24.8±5.7 27.1±7.5 15.5±6.5 14.7±3.5 16.0±3.5 20.4±7.8
100 mg/kg
AVIFS 0.0 07.5±4.7 17.0±6.1 18.6±11.4 16.9±10.9 11.1±11.1 08.9±4.8 10.9±5.5 26.1±6.6
100 mg/kg
AVIFZ 05.8±5.5 13.0±8.7 19.0±9.3 21.1±4.6 20.1±7.1 14.6±6.8 14.3±25.4 19.7±4.7 35.1±8.5
100 mg/kg
Avipattikara churna is normally given for a chronic period in treating hyperacidity, ulcer and related bowel disturbances. But in the present study only single bolus dose was given and observed the effect of the preparations was observed on only one model of hyperacidity. The effects of chronic administration on this model and on different models of hyperacidity are being planned. It is also planned to explore the possible mechanism of action of these preparations in reducing the gastric acid content. However the present study has indicated that OT and its marketed preparations (Avipattikara churna) are effective in reducing hyperacidity and related git disturbances. References

Ambasta S.P. (1986). The Useful Plants of India, Publications and Information directorate, New
Delhi CSIR, 409.
Ayurvedic Formulary of India, (1976). 1st edition, Ministry of Health and Family Planning,
Department of Health, Govt. of India, New Delhi, (I), 87.
Kulkarni S.K. (1987) Hand book of Experimental Pharmacology, Vallabh Prakashan, New
Delhi, 77.
Kulkarni S.K. (1987) Hand book of Experimental Pharmacology, Vallabh Prakashan, New
Delhi, 65.
Prabhakara Ogle (1931). Chikithsa Prabhakara, 2nd edn., Shankar Narahari Joshi, Pune, 394. Ram P. Rastogi and Mehrotra B.N. (1999). Compendium of Indian Medicinal Plants, Central drug Research Institute and New Delhi, National Institute of Science Communication, Lucknow, 2: 499. Robert A. Turner (1965). Screening methods in pharmacology, Academic Press, New York, 22. Robert A. Turner (1965). Screening methods in pharmacology, Academic Press, New York, 135. Shay H., Komarow S.A., Fles S.S., Meranze D., Gruenstein M. and Siplel H.A., Simple method for production of uniform gastric ulcers in rats. Gastroenterology (1945), 43. Received: 04.01.2005 Accepted: 30.12.2005

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