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Khoshboueilab.com

Dott. Damiano Angoli Researcher, Lab Manager email: [email protected] Citizenship: Canadian, Italian.
Education and Languages:
* 1988-94 Laurea (Doctor in Biological Sciences) with honor (110/110) in Biology at Dept. of
Biochemistry and General Physiology, Electrophysiology Laboratory, University of Milan, Italy.
“Regulation of intracellular pH in human promyelocytic leukemia cells”.
Supervisor: Prof. Enzo Wanke.
* 1986-87 Diploma in Computer Program Development at University del Sacro Cuore of
Milan.
* other certifications: Microsoft office Specialist
* Languages: English (spoken very good, reading and written good). French (speaking and
reading good, writing fair). Italian (fluent).
Related Work Experience:
* Oct 2005-Dec 2012 Researcher Lab Manager, Dept. of Cellular & Physiological Sciences
Life Sciences Centre - 2350 Health Sciences Mall
The University of British Columbia
Vancouver, BC, Canada
* 2000-sept 2005 Post-secondary Research Assistant.
Ion Channel Laboratory, School of Kinesiology, Simon Fraser University.
Burnaby BC, Canada
* 1998-2000 Independent web design for several companies.
* 1997-99 Worked at Pfizer Italiana as Healthcare Representative, responsible for calling on
practicing physicians and hospitals to provide information about Pfizer’s products such as
Norvasc, Viagra, Feldene, Zoloft, and Diflucan.
Personnel director: Dr. Saverio Martini
* 1995-96 Fellowship in cellular physiology funded by Italian Telethon . Dept. of Biochemistry
and General Physiology, Electrophysiology Lab, University of Milan, Italy in collaboration with
the Besta Neurological Institute. “Adhesion properties of human (Duchenne) and murine (mdx)
dystrophic myotubes”
P.I.: Prof. Enzo Wanke.

* 1988-94 Self-employed as a programmer for computer-driven tool machines used to work and
design metal.
Experimental and Technical Skills:
* Strong cellular biology skills: primary and cell lines in culture, ion channel transfection
techniques, immunocytochemistry. Specific work with the pacemaker channel protein.
* Patch clamp electrophysiology and analysis using the Axon/pClamp, Microcal Origin,
Microsoft software, Axon 200B amplifier and associated hardware. Specifically analysis of the
pacemaker channel proteins.
* Confocal Imaging using the Zeiss Pascal microscope and associated imaging software.
Specific work with the pacemaker channel protein.
* Skills in common Microsoft products, Macromedia products (for web sites
design/implementation) and Adobe programs for editing, publishing and analyzing data, word,
excel, power point, photoshop, illustrator, dreamweaver, flash, fireworks, midi files, audio-video
editing, freehands, autocad and other graphics software, and software for computer-driven tool
machines.
* Preparing data for oral, video and poster presentations at international and/or local meetings.
* Skills in assembling and handling computer hardware
* Putting together, designing and machining, solving problems in new or existing experimental
set-ups
* Organizing the normal working day in the lab, including supervision and teaching of students,
organization of experiments.
* Computer programming (from Diploma in Computer Programming Development)

Other:
Editor for the American Journal of Physiology-Cell Physiology, since 2009.
Published Manuscripts:
Macri V, Angoli D, AccilSci Rep. 2012;2:894. doi: 10.1038/srep00894. Epub 2012 Nov 27.
Hegle AP, Nazzari H, Roth A, Angoli D, AccilAm J Physiol Cell Physiol. 2010
May;298(5):C1066-76. Epub 2010 Feb 3.
Peters CJ, Chow SS, Angoli D, Nazzari H, Cayabyab FS, Morshedian A, AccilJ
Mol Cell Cardiol. 2009 May;46(5):636-43. Epub 2009 Jan 27.
Nazzari H, Angoli D, Chow SS, Whitaker G, Leclair L, McDonald E, Macri V, Zahynacz K,
Walker V, Accili
Am J Physiol Cell Physiol. 2008 Sep;295(3):C642-52. Epub 2008 Jul 9.
Whitaker GM, Angoli D, Nazzari H, Shigemoto R, Accili EA. HCN2 and HCN4 isoforms
self-assemble and co-assemble with equal preference to form functional pacemaker channels.
J Biol Chem. 2007 Aug 3;282(31):22900-9. Epub 2007 Jun 6.
Macri V, Proenza C, Agranovich E, Angoli D, Accili EA. Separable gating mechanisms in a
Mammalian pacemaker channel. J Biol Chem. 2002 Sep 27;277(39):35939-46.
Proenza C, Tran N, Angoli D, Zahynacz K, Balcar P, Accili EA. Different roles for the cyclic
nucleotide binding domain and amino terminus in assembly and expression of
hyperpolarization-activated, cyclic nucleotide-gated channels. J Biol Chem. 2002 Aug
16;277(33):29634-42.
Proenza C, Angoli D, Agranovich E, Macri V, Accili EA. Pacemaker channels produce an
instantaneous current. J Biol Chem. 2002 Feb 15;277(7):5101-9
Angoli D, Corona P, Baresi R, Mora M, Wanke E. Laminin-alpha2 but not -alpha1-mediated
adhesion of human (Duchenne) and murine (mdx) dystrophic myotubes is seriously defective.
FEBS Lett. 1997 May 26;408(3):341-4.
Angoli D, Delia D, Wanke E. Early cytoplasmic acidification in retinamide-mediated apoptosis of
human promyelocytic leukemia cells. Biochem Biophys Res Commun. 1996 Dec
13;229(2):681-5.
Published Abstracts:
R Yip , D Angoli, C Ahern, EA Accili. Lidocaine Inhibition of Hyperpolarization-activated
Cyclic Nucleotide-modulated Channel Isoform 1 (HCN1) is Fast, Voltage-dependent and
Reversible. American Biophysical Society, 2009.
R Yip , D Angoli, EA Accili. Lidocaine inhibits cloned Hyperpolarization-activated Cyclic
Nucleotide-gated channel isoform 1 (HCN1). American Biophysical Society, 2008.
D Angoli, G Whitaker, F Cayabyab,V Macri, EA Accili. A novel role for ion channel
heteromerization. American Biophysical Society, 2004.
D Angoli, G Nalewajek, V Macri, F Cayabyab, EA Accili. HCN2 and HCN4 form
heteromultimers in CHO cells. Role of the N-terminus. Biophysical Journal, Mar 2003.
K Zahynacz, D Angoli, C Proenza, V Macri, EA Accili. A conserved region of the CNBD is
required for functional expression of HCN channels. Biophysical Journal, Mar 2002.
C Proenza, N Tran, D Angoli, K Zahynacz, EA Accili. Role of the cyclic nucleotide-binding
domain (CNBD) in functional expression of HCN channels. Biophysical Journal, Feb 2002.
C Proenza, D Angoli, EA Accili. Pacemaker channels induce a cAMP sensitive background
current. Biophysical Journal, Feb 2002.
D Angoli, L. Faravelli, E Wanke. Integrin-independent adhesion of normal and mdx myotubes on
laminin-coated substrates. Pflugers Archives 434, 1997.
D Angoli, D Delia, E Wanke. Cytoplasmic acidification is an early event of retinoid-induced
apoptosis in human malignant leukemic cells. Pflugers Archives 431, 1996.
Presentations:
D Angoli, G Whitaker, F Cayabyab,V Macri, EA Accili. A novel role for ion channel
heteromerization. Poster for the American Biophysical Society, Long Beach CA, 2004.
D Angoli, G Nalewajek, V Macri, F Cayabyab, EA Accili. HCN2 and HCN4 form
heteromultimers in CHO cells. Role of the N-terminus. Poster for the American Biophysical
Society, San Antonio Texas, Mar 2003.
K Zahynacz, D Angoli, C Proenza, V Macri, EA Accili. A conserved region of the CNBD is
required for functional expression of HCN channels. Poster for the American Biophysical Society,
San Francisco, CA Mar 2002.
D Angoli, L. Faravelli, E Wanke. Integrin-independent adhesion of normal and mdx myotubes on
laminin-coated substrates. Oral presentation, Italian Physiological Society, Florence, Italy, 1996
(prepared by me, presented by E. Wanke).
D Angoli, D Delia, E Wanke. Cytoplasmic acidification is an early event of retinoid-induced
apoptosis in human malignant leukemic cells. Oral presentation, Italian Physiological
Society, Florence, Italy, 1995

Referees:

Eric Accili PhD, Associate Professor.
Dept. of Cellular & Physiological Sciences
Life Sciences Centre - 2350 Health Sciences Mall
The University of British Columbia
Vancouver, BC V6T 1Z3 Canada
Phone: 001 (604) 822-6900
Fax: 001 (604) 822-2316
[email protected]
Catherine Proenza, PhD
Assistant Professor
University of Colorado School of Medicine
Department of Physiology & Biophysics
12800 E 19th Ave, MS8307
Aurora, CO 80045 303-724-2522 (phone) 303-724-4501 (fax) [email protected] Prof. Enzo Wanke Dipartimento di Biotecnologie e Bioscienze Università di Milano-Bicocca Piazza della Scienza, 2 20126 MILANO, Italy Tel. 39 02 644 83303 [email protected] Prof. Achille Ghidoni Ordinario di Citogenetica (retired), Università degli Studi dell’Insubria, Via Ravasi, 2 - 21100 Varese, Italy. Tel 39 0331 021548 [email protected]

Source: http://www.khoshboueilab.com/sites/default/files/CV.pdf

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