Microsoft word - cv henrique silveira-ingles-jan13.doc
Name: HENRIQUE Manuel Condinho da SILVEIRA Date of birth: 28/04/1966 Marital status: Married, 2 daughters Nationality: Portuguese Institutional address: Centro de Malaria e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira 100, 1349-008 Lisboa Portugal Home address: Av Duque de Loule, 50, R/C, 1050-091Lisboa Tel: (+) 351-916800174 Fax: (+) 351 21 3632135 Email:[email protected] Academic degrees: 2008 “Agregação” in Medical Parasitology. Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Portugal..
1994 PhD in Parasitology. Imperial College, University of London, UK. Supervised by Prof.
Elisabeth U. Canning entitled "In vitro and in vivo models for development and chemotherapy of human microsporidia". Supported by "Programa Ciência - JNICT", Portugal
1994 Diploma of the Imperial College 1989 Four-year degree in Biology. Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto (UP), Portugal.
Areas of interest (key words): Health Sciences - Entomology, parasitology, malaria, molecular biology, vector refractoriness to infection, immunology Areas of activity: The research of my group is centered on the mosquito response to the infection and how this response can be modulated by external factors. Our goal is to develop effective tools to prevent malaria transmission by Anopheles mosquitoes. In the recent past we have clearly established that cloroquine can alter mosquito response to parasite, contributing to differences on the parasite sporogonic development. Chloroquine acts not only at classical immune response pathways but also interferes with the expression of genes associated with apoptosis and micro-skeleton, which are major players for containment of parasite invasion. Among the different player on the response to the infection we have shown that antioxidant proteins have an important role on the response to the infection and could be associated with genes previously linked to insecticide resistance. At the moment we are investigating the use of immunostimulatory molecules to boost mosquito response to parasite in order to increase resistance to infection and subsequently drop parasite numbers under transmission threshold. A similar approach, but using mosquitoes collected in the field and experimentally infected with Plasmodium vivax will be developed in Manaus, Brazil. We also use Anopheles gambiae, and Anopheles arabiensis mosquitoes collected in Africa to access immune-related genes variability and the impact of infection on their evolution. To develop our research we have extensive experience in genomic, proteomic and gene silencing tools to study mosquitoes. We use our facilities to rear different species of mosquitoes, and fully control the mosquito life cycle. We have used A. gambiae, Anopheles stephensi, Anopheles albimanus as models in our studies. We also participate in activities in the field of placental malaria in Angola, under collaboration with Paulo Adão Campos. At the Tropical Medicine Foundation Dr º Heitor Vieira Dourado, Manaus, Amazonas, Brazil we also develop activities on the molecular characterization of Trypanosoma cruzi from the Amazon and its association with the eco-biology of vectors and other vertebrate hosts. The research group is composed of a postdoc, a PhD student, two project fellows and a graduate students.
Present position, institution, starting date: 2012/
Associate Professor, Instituto de Higiene e Medicina Tropical, UNL
Previous positions: 2001/12
Assistant Professor, Instituto de Higiene e Medicina Tropical, UNL
Visiting Researcher at The Foundation of Tropical Medicine in the Amazons (FMTAM), Manaus, Brazil. Supported by grant of the FAPEAM, Brazil.
Invited Assistant Professor at IHMT, UNL
Post-Doctoral Grant Praxis XXI (BPD/11781/97) at the CMDT/IHMT, in Prof. Virgilio E. do Rosário Laboratory. “The influence of the human immune response on the sporogonic cycle of
Plasmodium in the mosquito vector: Effect on Circumsporozoite gene expression and parasite selection”.
Research Fellow at the London School of Hygiene and Tropical Medicine, UK. (EU funded, trough Prof. D. Bradley) “Plasmodium falciparum malaria diversity of T-cell epitope polymorphisms of the circumsporozoite protein of parasites isolated from children in The Gambia” The work was developed at the laboratories of Prof. Brian Greenwood, Prof. Geoffrey Targett and Dr. David Conway.
Post doctoral position in the project "Detrimental effects of TB on natural history of HIV infection: Viral load, immune function and apoptosis", TS3-CT93-0245, funded by the European Union and Co-ordinated by Prof. F. Ventura (at CMDT)
Secondary School Teacher at Escola Secundária Homem-Cristo, Aveiro.
Monitor at the Cellular Biology Department at ICBAS, UP.
Supervision of students:
• PhD students: 6 • MSc students: 9 • Graduation students: 8
Organization of international courses (and teaching)
• Biotechnology Applied to Infectious and Parasitic Diseases. Post-graduate programme FMTAM/UEA, Manaus, AM, Brazil (annually - 2008 till 2011). • COST 857 Transfection workshop. Lisbon, 19-22nd October 2008. • Advances in Molecular and Cellular Biology. Post-graduate programme RENORBIO, Teresina, PI, Brazil (annually - 2008 till 2011). • “Laboratory Animal Sciences” (equivalent to Cat C – FELASA), IHMT, Lisboa, 17-28/04/06. Co-
organization with Dr. Olson A. (IBMC, University of Porto), • “Methodologies of Research using molecular biology tools” National Institute of Health, Luanda, Angola 20-23/03/06. • "Molecular tools applied to health and environmental studies", Biotecnology Center and
Veterinary School, University Eduardo Mondlane (CB and Vet Fac, UEM) Maputo, Mozambique 25-29/07/2005. • “Principals of genetics and molecular biology applied to health” National Institute of Health, Luanda, Angola 27-04/06/05. • “Gene Silencing” University of Algarve 12-15/04/05. • “Informatic tools applied to research in molecular biology”, Centro de Biotecnologia da
Universidade Eduardo Mondlane, Maputo, Mozambique 07– 10/06/2004 (co-organiser with Luis Neves, UEM, Mozambique and Sonia Centeno-Lima, CMDT, Portugal)
• Coordinator of the “Molecular Parasitology” module from the MSc and PhD programmes on
Biomedical Sciences. (TropEd accredited course) • Post –Graduate Course on Medical Parasitology, IHMT, UNL - 1996/1997 till present. • Master on Medical Parasitology, IHMT, UNL – 1996/1998 till present. • Graduate programme in areas of Basic and Applied Biology - GABBA, Porto University -
1999/2000 till present • Master on Immunology ICBAS, Porto University (1996/1998) • “Immunity and Parasitism” IGC (Gulbenkian Institute of Science), Lisbon 12-16/07/1999 and
29/05-02/06/00. • “Improved Training and Research in Community Health related to the Control of Vector Borne
Diseases”, Trujillo Venezuela, 7-12/06/1999. (ALFA programme). • “Identification of malaria genotypes” on the “IV Course on Tropical Medicine and Parasitology”
Tropical Medicine and Parasitology UNESCO Professorship, Granada University. 21/09/98 – 23/10/98.
• Workshop on transfection of malaria parasites, New Delhi, India 15 - 21 April 2002, International
Centre for Genetic Engeneering and Biotechnology (ICGEB).
Organized by Prof A Cowman, Dr. B. Crabb, from The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia, and Profs. V. Chahaum, C. Chitnis e P. Malhotra from the ICGEB. The workshop was financed by WHO/TDR e MR4. H.S. participation was financed by Fundação Caloust Gulbenkian.
• Veterinary Pathobiology Department, University of Minnesota, USA, at the Dr. Mitchell
Abrahamsen Laboratory. Finnanced by FLAD, Fundação Luso Americana para o Desenvolvimento and ICCTI, Instituto de Cooperação Ciência e Tecnologia Internacional. August - November 2000.
On differential display of mRNA (DDRT-PCR) and Serial Analyses of Gene Expression (SAGE) technology for the identification and characterisation of Plasmodium genes that are involved on immune/drug evasion during the sporogonic development in the mosquito vector.
• Unité D’Immunochimie Analytique, Institut Pasteur, Paris, França (Director Pierre-André
Cazenave). Financed by ICCTI/CNRS bilateral agreement. 6-26 June 2000.
To test the applicability of Immunoscope (PE Byosystems) methodology to compare T cell receptor β chain variable regions (TCRB) in placenta and in peripheral blood of malarious and
• Instituto de Inmunologia del Valle, Universidad del Valle, Cali, Colombia at Prof. Socrates Herrera
Laboratory and Instituto de Ciências Biomédicas, Universidade São Paulo (USP), Brazil at Prof. Hernando del Portillo. November 1997 and February 1998.
Development of protocols for transport and manipulation of Plasmodium falciparum RNA, from experimental infections of the mosquito Anopheles albimanus.
Financed projects as PI
• Immune stimulation of the mosquito Anopheles gambiae response against the malaria parasite
Plasmodium berghei PTDC/SAU-MII/102596/2008 (2010-2014) • Gene variability of pattern-recognition-molecules (PRM) involved in mosquito immune response
to Plasmodium POCI/SAU-IMI/59489/2004 (2005-2009) • The effect of chloroquine on the modulation of mosquito vector response to Plasmodium
infection. ref POCTI/MGI/344905/2002 (2003-2006) • Studies on the effect of specific anti-Plasmodia antibodies on the development of the parasite and
gene expression in the mosquito vector, ref. POCTI/ESP/43636/99 (2001-2003) • Plasmodium gene expression during the sporogonic development in the mosquito vector: effect of
specific antibodies and antimalarial drugs. ref. POCTI/MGI/35815/00 (2001-2004) • Imunofisiopatologia da malária placentar humana: comparação entre os repertórios de linfócitos
T placentares e circulantes (Immunophysiopathologie du paludisme placentaire humain: comparaison des répertoires des lymphocytes T circulants et placentaires). Acordo bilateral ICCTI/CNRS proc. 423. (2000-2001) • Study of Interleukins 6, 10 and 12 on Plasmodium and Leishmania infections
Financed projects as Team member
• Protection against malaria by “natural” Antibodies - Bill & Melinda Gates Foundation – Grand
Challenges (PI: Miguel Soares, IGC) • Screening of potential molecular markers for stage determination in Human African
Trypanosomiasis. - PTDC/SAU-ESA/102435/2008 (PI: Nuno Rolão, UEICDT, CMDT, IHMT, UNL) • European research Infrastructure on Highly Pathogenic agents - ERINHA (IHMT Associate
Member –Henrique Silveira) • Consequences of neutrophil-Leishmania infantum interactions in the establishment of canine
Leishmaniasis. - PTDC/CVT/113121/2009 (PI: Gabriela Santos Gomes, UEIPM, CMDT, IHMT, UNL) • Factors affecting dynamics of Plasmodium concomitant infections in a malaria endemic area:
analysis through a new modelling approach? - PTDC/SAU-EPI/113326/2009 – (PI: Ana Paula Arez, UEIPM, CMDT, IHMT, UNL)
• Research capacity for the implementation of genetic control of mosquitoes – INFRAVEC – (PI:
João Pinto, UEIPM, CMDT, IHMT, UNL) • Functional characterisation of orphan family 2 GPCR receptors in the malaria model – PTDC
/BIA-BC M/114395/2009 (PI: João Cardoso, Centre of Marine Sciences, U. Alg)
• Evaluator of STSM grants from COST Action 857 - Apicomplexan Biology in the post-genomic
era (2004 to 2009). • Member of the scientific expert evaluators panel, Life Science, Marie Curie European Fellowships
(2004 - 2006). • Member of the scientific expert evaluators panel under the INCO specific measures for the call
FP6-2004-INCO-DEV-3 (2005). • Scientific Revision of manuscripts for BMC Genomics • Member of PhD and Msc examination panels (in Portugal and Brasil) • Member of several evaluation panels for IHMT PhD/Research student selection and Selection
committee of the Post-graduate programme of Infectious and Parasitic Diseases, FMT-AM e UEA, Manaus, Brazil.
• Elected Scientific Coordinator of the Associate Laboratory – Centre for Malaria Studies (CMDT)
of the Science Foundation, Ministry of Science and higher education, Portugal (2011-2013) • Deputy Director from the IHMT, UNL (2011-2013) • Coordinator of the PhD program on Biomedical Sciences, IHMT, UNL (2010 till present) • Portuguese delegate to the managing comity of COST 857 – “Apicomplexan biology in the post-
genomic era” • Elected member of the Scientific Committee of the IHMT, UNL. • Local organiser of the COST Action 857 - Apicomplexan Biology in the post-genomic era, 1st
Annual Workshop, Lisbon, Portugal, 3-6/05/2004 • ICCTI delegate in the Workshop - Health and Quality of life, ALCUE (EU-Latin America &
Caribean S&T Cooperatioon), Rio de Janeiro 7/10-11-2001. • Visit to several US Institutions, sponsored by the US International Agency - International visitor
program Co-operation in fighting Infectious Disease” 13-24/09/1999.
Publications in international peer-reviewed journals:
Monteiro WM, Margioto Teston AP, Gruendling AP, dos Reis D, Gomes ML, de Araújo SM, Bahia
MT, Magalhães LKC, Guerra, JAO, Silveira H, Toledo MJO, Barbosa MGV. 2013. Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice. PLoS Negl Trop Dis 7(2): e2069. Silveira H, Gabriel A, Ramos S, Palma J, Felix R, Custodio A, Collins V. 2012. CpG-containing
oligodeoxynucleotides increases resistance of Anopheles mosquitoes to Plasmodium infection. Insect Biochem Mol Biol 42:758-765.
Monteiro WM, Magalhães LKC, de Sá ARN, Gomes ML, Toledo MJdO, L, Pires I, Guerra JAO,
Silveira H, Barbosa MGV. 2012. Trypanosoma cruzi IV Causing Outbreaks of Acute Chagas Disease and Infections by Different Haplotypes in the Western Brazilian Amazonia. PLoS ONE 7: e41284.
Monteiro WM, Magalhães LK, Oliveira JC, Guerra JA, Silveira H, Ferreira LC, Toledo MJ, Barbosa
Md. 2012. Biological behavior of Trypanosoma cruzi stocks obtained from the State of Amazonas, Western Brazilian Amazon, in mice. Rev Soc Bras Med Trop. 45:209-14.
Campos PA, Valente B, Campos RB, Goncalves L; do Rosario VE, Varandas L, Silveira H. 2011
Prevalence and risk factors of Plasmodium falciparum infection in pregnant women attending prenatal visits in Luanda. Rev Soc Bras Med Trop. 45:369-74.
Félix RC, Silveira H. 2011. The Interplay between Tubulins and P450 Cytochromes during
Plasmodium berghei Invasion of Anopheles gambiae Midgut. PLoS ONE 6: e24181.
Valente B, Campos PA, do Rosário VE, Silveira H. 2011. Prevalence and risk factors of Plasmodium
falciparum infections in pregnant women in Luanda, Angola. Trop Med Int Health. 16: 1206–1214.
Guerra JA, Prestes SR, Silveira H, Coelho LI, Gama P, Moura A, Amato V, Barbosa MG, Ferreira
LC. 2011. Mucosal Leishmaniasis caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon. PLoS Negl Trop Dis. 5(3):e980.
Valente B, Campos PA, do Rosário VE, Silveira H. 2010. Natural frequency of polymorphisms linked
to the Chondroitin 4-Sulfotransferase genes and its association with placental malaria. Trans R Soc Trop Med Hyg. 104:687-9.
Monteiro WM, Magalhães LKC, Simões F, Medeiros, MB, Silveira H, Barbosa MGV. 2010.
Trypanosoma cruzi TCIII/Z3 genotype as agent of an outbreak of Chagas disease in the Brazilian Western Amazonia. Trop Med Int Health. 15:1049.
Félix RC, Müller P, Ribeiro V, Ranson H, Silveira H. 2010. Plasmodium infection alters Anopheles
gambiae detoxification gene expression. BMC genomics. 19;11(1):312.
Mendes C, Felix R, Sousa AM, Lamego J, Charlwood D, do Rosario VE, Pinto J, Silveira H. 2010.
Molecular evolution of the three short PGRPs of the malaria vectors Anopheles gambiae and Anopheles arabiensis in East Africa. BMC Evol Biol. 12;10(1):9.
Caldeira RL, Gonçalves LM, Martins TM, Silveira H, Novo C, Rosário V, Domingos A. 2009.
Plasmodium chabaudi: expression of active recombinant chabaupain-1 and localization studies in Anopheles sp. Exp Parasitol. 122:97-105.
Abrantes P, Dimopoulos G, Grosso AR, do Rosário VE, Silveira H. 2008. Chloroquine mediated
modulation of Anopheles gambiae gene expression. PLoS ONE. 3(7):e2587.
Silveira H, Ramos S, Abrantes P, Lopes LF, do Rosario VE, Abrahamsen MS. 2007. Effect of
chloroquine on gene expression of Plasmodium yoelii nigeriensis during its sporogonic development in the mosquito vector. Malar J. 6:84.
Lopes, LF; Zamora, F; Rocha, L, Marques PX, Mendes C, Martinez-Barnetche J, Leandro MJ,
Arevalo M, Herrera S, do Rosario VE, Arez AP, Silveira H. 2007. Anopheles albimanus immune response to single and mixed infections with Plasmodium falciparum and Plasmodium vivax. Trop Med Int Health. 15 (Suppl 1):201 (Abstract)
Lopes, LF, Abrantes P, Silva AP, do Rosario VE, Silveira H. 2007. Plasmodium yoelii: the effect of
second blood meal and 1 anti-sporozoite antibodies on development and gene expression in the mosquito vector, Anopheles stephensi. Exp. Parasitol 115:259-69.
Lobo ML, Silveira H, Ramos S, Xiao L, Matos O. 2006. Characterization of a pathogen related to
Vavraia culicis detected in a laboratory colony of Anopheles stephensi. J Eukaryot Microbiol. 53 (Suppl 1):S65-7.
Jesuíno BS, Casimiro C, do Rosário VE, Silveira H. 2006. Effect of antibodies on the expression of
Plasmodium falciparum circumsporozoite protein gene. Int J Med Sci 3:10-13.
Marques CS, Rolão N, Centeno-Lima S, Lousada H, Maia C, Campino L, do Rosário VE, Silveira H.
2005. Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: IFN-g and IL-4 mRNA expression. Mem Inst Oswaldo Cruz 100: 889-892
Abrantes P, Lopes LF, do Rosário, VE, Silveira H. 2005. Effect of chloroquine on the expression of
genes involved on the mosquito response to Plasmodium infection. Insect Biochem Mol Biol 35:1124-1132
Ordway D, Costa L, Martins M, Silveira H, Arroz MJ, Ventura F, Dockrell H. 2004. Increased IL-4
response to virulent Mycobacterium tuberculosis in health care workers, who went on to present with primary tuberculosis. J Infec Diseases 190:756-766
Franco AS, Silveira H, do Rosário VE. 2003. Plasmodium yoelii: Semi-quantitative analyzes of
circumsporozoite protein gene expression during the sporogonic development of P.y. yoelii and P. y. nigeriensis in the mosquito vector, Anopheles stephensi. J Parasitol 89: 255-260. Silveira H, Dias CR, do Rosario VE. 2001. Single strand conformational polymorphism evaluation for
the identification of TH3R alleles of the circumsporozoite protein gene of Plasmodium falciparum. Parassitologia 43: 155-157.
Santos-Gomes G, Rosa R, Leandro C, Cortes S, Romão P, Silveira H. 2002. Cytokine expression
during the outcome of canine experimental infection by Leishmania infantum. Vet Immunol Immunopathol 88:21-30
Centeno-Lima S, Silveira H, Casimiro C, Aguiar P, do Rosário VE. 2002. Kinetics of cytokine
expression in mice with invasive aspergillosis: lethal infection and protection. FEMS Immunol Med Microbiol 32:167-173.
Fernandes NEP, Silveira H, Franco AS, Arez APA, Forte JM, do Rosário VE. 2001. Detection of
malaria parasites, in paraffin-embedded spleen and placental tissues, by nested PCR. Trans R Soc Trop Med Hyg 95:293-294.
Santos-Gomes GM, Leandro C, Rosa R, Silveira H, Cortes S, Campino L. 2001. L. infantum infection
in canine model. Humoral and cellular immune responses. In: Canine Leishmaniosis: treatment and follow-up. Ed.Cost Directorate-General Research, Madrid, 25-29
Alloueche A, Silveira H, Conway DJ, Bojang K, Doherty T, Cohen J, Pinder M, Greenwood BM 2000.
High-throughput sequence typing of T-cell epitope polymorphisms in Plasmodium falciparum circumsporozoite protein. Mol Biochem Parasitol 106:273-282.
Rosa R, Silveira H, Seixas E, Rolão N, Santos-Gomes G, do Rosario VE 1999 The effect of
chloroquine on the production of IFN-γ, IL-4, IL-6 and IL-10 in Plasmodium chabaudi chabaudi AS
infected C57BL6 mice. J. Parasitol. 85:956-960. Silveira H, Ordway D, Jackson M, Dockrell H, Ventura F 1997. Cell mediated immune responses to
mycobacterial antigens in patients with pulmonary tuberculosis and human immunodeficiency virus infection. Clin. Exp. Immunol. 110, 26-34. Silveira H, Canning EU 1995. In vitro cultivation of the human microsporidium Vittaforma corneae:
development and effect of albendazole. Folia Parsitol. 42, 241-250. Silveira H, Canning EU 1995. Vittaforma corneae N.Comb. for the human microsporidium Nosema
corneum Shadduck Meccoli, Davis & Font, 1990 based on its ultrastructure in the liver of experimentally infected athymic mice. J. Euk. Microb., 42, 158-165. Silveira H 1994. In vitro and in vivo models for development and chemotherapy of human
microsporidia. PhD Thesis. Imperial College of Science Technology and Medicine, University of London.
Silveira H, Canning EU 1994. Ultrastructure evidence for a new generic emplacement for Nosema
corneum, a microsporidian parasite of the human cornea Trans R Soc Trop Med. 88, 376. (Abstract) Silveira H, Canning EU & Shadduck, JA 1993. Experimental infection of athymic mice with the human
microsporidian Nosema corneum. Parasitology, 107, 489-496. Silveira H, Rodrigues, P, Azevedo, C 1990. Fine structure of the spermatogenesis of Blennius pholis
(Pisces, Blenniidae). J Submicrosc Cytol Pathol. 22, 103-108.
Pipe, RK, da Silveira HMC 1989. Arylsulphatase and acid phosphatase activity associated with
developing and ripe spermatozoa of the mussel Mytilus edulis. Histochem. J., 21, 23-32. Publications in peer-reviewed journals (Portuguese): Campos PA, Valente B, do Rosário VE, Varandas L, Silveira H. 2010. Caracterização da malária
placentária por Plasmodium falciparum em Luanda, Angola. Ciência e Tecnologia, 2, 39-50.
Abrantes P, Silveira H. 2009. Alterações climáticas na Europa: efeito nas doenças parasitárias
humanas - Climate change in Europe: impact on human parasitic diseases. Revista Portuguesa de Saúde Pública. 27(2): 71-86.
Ordway D, Silveira H, Costa L, Costa SL, Martins M, Badura R, Arroz MJ, Young S, Dockrell H,
Ventura F. 2002. Comparação das respostas celulares imunes induzidas por proteinas filtradas da cultura de Mycobacterium tuberculosis. Revista Portuguesa de Pneumologia 8: 629-643.
Book Chapters Félix RC, Silveira H. 2012. The Role of Anopheles gambiae P450 Cytochrome in Insecticide
Resistance and Infection In: Insecticides - Pest Engineering. Ed. Perveen F. InTech - Open Access Publisher (ISBN 978-953-307-895-3)
Lyme Disease - frequently asked questions What is Lyme disease? It is an infection caused by a spiral shaped bacterium called Borrelia burgdorferi that is transmitted to humans by bites from infected ticks. The infection is not transmitted directly from person to person, nor from the bites of other types of insect, nor from animals. What symptoms can it cause? The most common and
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